Caenorhabditis elegans is an instrumental analysis mannequin used to advance our data in areas together with growth, metabolism, and getting old. However, analysis on metabolism and/or different measures of well being/getting old are confounded by the nematode’s food source in the lab, reside E. coli micro organism. Commonly used therapies, together with ultraviolet irradiation and antibiotics, are profitable in stopping bacterial replication, however the micro organism can stay metabolically energetic. The goal of this examine is to develop a metabolically inactive food source for the worms that may enable us to attenuate the confounding results of bacterial metabolism on worm metabolism and getting old.
Our technique is to make use of a paraformaldehyde (PFA) handled E. coli food source and to find out its results on worm well being, metabolism and longevity. We initially decide the bottom potential concentrations of PFA essential to quickly and reproducibly kill micro organism. We then measure numerous points of worm conduct, well beingspan and longevity, together with progress price, food attraction, brood measurement, lifespan and metabolic assessments, comparable to oxygen consumption and metabolomics. Our ensuing knowledge present that worms eat and develop effectively on these micro organism and assist the usage of 0.5% PFA-killed micro organism as a nematode food source for metabolic, drug, and longevity experiments.
Primary goal 1. To assess the consequences of offering grownup sufferers with entry to digital well being information (EHRs) alone or with extra functionalities on a variety of affected person, patient-provider, and well being useful resource consumption outcomes, together with affected person data and understanding, affected person empowerment, affected person adherence, affected person satisfaction with care, adversarial occasions, well being-related high quality of life, well being-related outcomes, psychosocial well being outcomes, well being useful resource consumption, and patient-provider communication. Secondary goal 1. To assess whether or not results of offering grownup sufferers with EHR entry alone versus EHR entry with extra functionalities differ amongst affected person teams in line with age, academic degree, or completely different standing of illness (power or acute).
Adult affected person entry to digital well being information
We included 10 studies with 78 to 4500 members and follow-up from three to 24 months. Nine studies assessed the consequences of EHR with extra functionalities, every addressing a subset of outcomes sought by this assessment. Five studies targeted on sufferers with diabetes mellitus, 4 on sufferers with particular ailments, and one on all sufferers. All studies in contrast EHR entry alone or with extra functionalities plus normal care versus normal care solely. No studies assessing the consequences of EHR entry alone versus EHR entry with extra functionalities had been recognized.
Interventions required a wide range of knowledge throughout the EHR, comparable to affected person historical past, drawback record, treatment, allergy symptoms, and lab outcomes. In addition to EHR entry, eight studies allowed sufferers to share self-documented knowledge, seven provided individualised illness administration features, seven provided academic disease-related info, six supported safe communication, and one provided preventive reminders. Only two studies had been at low or unclear threat of bias throughout domains. Meta-analysis couldn’t be carried out, as members, interventions, and outcomes had been too heterogeneous, and most studies introduced outcomes primarily based on completely different adjustment strategies or variables.
The high quality of proof was rated as low or very low throughout outcomes. Overall variations between intervention and management teams, if any, had been small. The relevance of any small results stays unclear for most outcomes as a result of in most instances, trial authors didn’t outline a minimal clinically essential distinction. Overall, outcomes recommend that the consequences of EHR entry alone and with extra functionalities are principally unsure compared with normal care. Patient data and understanding: very low-quality proof is offered from one examine, so we’re unsure about results of the intervention on affected person data about diabetes and blood glucose testing.
Patient empowerment: low-quality proof from three studies means that the intervention might have little or no impact on affected person empowerment measures. Patient adherence: low-quality proof from two studies means that the intervention might barely enhance adherence to the method of monitoring threat components and preventive providers. Effects on treatment adherence are conflicting in two studies; this will likely or might not enhance to a clinically related diploma. Patient satisfaction with care: low-quality proof from three studies means that the intervention might have little or no impact on affected person satisfaction, with conflicting outcomes.
 Protein) Recombinant Rat MIP-1 Beta (CCL4) Protein |
|
PROTP50230-1 |
BosterBio |
20ug |
EUR 317 |
|
Description: Both MIP-1α and MIP-1β are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1α and MIP-1β exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1α selectively attracting CD8+ lymphocytes and MIP-1β selectively attracting CD4+ lymphocytes. Additionally, MIP-1α and MIP-1β have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. Recombinant rat MIP-1β is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
 Protein) Recombinant Murine MIP-1 Beta (CCL4) Protein |
|
PROTP14097-1 |
BosterBio |
10ug |
EUR 317 |
|
Description: Both MIP-1α and MIP-1β are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1α and MIP-1β exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1α selectively attracting CD8+ lymphocytes and MIP-1β selectively attracting CD4+ lymphocytes. Additionally, MIP-1α and MIP-1β have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. Recombinant murine MIP-1β is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
) MIP-1 Macrophage Inflammatory Protein-1 Gamma Mouse Recombinant Protein (CCL9) |
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PROTP51670 |
BosterBio |
Regular: 20ug |
EUR 317 |
|
Description: MIP-1 gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 101 amino acids and having a molecular mass of 11.6 kDa. The MIP-1 gamma is purified by proprietary chromatographic techniques. |
 Anti-MIP-1 alpha Antibody |
|
A00405 |
BosterBio |
100ug |
EUR 432 |
|
Description: Rabbit Polyclonal MIP-1 alpha Antibody. Validated in WB and tested in Mouse. |
 Mouse CCL9/Mip 1 Gamma PicoKine™ Fast ELISA Kit |
|
FEK1225 |
BosterBio |
96 wells |
EUR 475 |
|
Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Mip 1 Gamma/Ccl9 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip 1 Gamma/Ccl9 tissue specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detected in a wide variety of tissues except brain. |
) MIP-1a Macrophage Inflammatory Protein-1 Alpha Mouse Recombinant Protein (CCL3) |
|
PROTP10855-1 |
BosterBio |
Regular: 10ug |
EUR 317 |
|
Description: Macrophage Inflammatory Protein-1 alpha Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7820 Dalton. ;The MIP-1a is purified by proprietary chromatographic techniques. |
 Anti-MIP antibody |
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STJ24556 |
St John's Laboratory |
100 µl |
EUR 277 |
|
Description: Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13. |
 anti- MIP antibody |
|
FNab05193 |
FN Test |
100µg |
EUR 548.75 |
|
|
|
Description: Antibody raised against MIP |
 Anti-NEMO/IKK-gamma Antibody |
|
A00874-1 |
BosterBio |
100ug |
EUR 455 |
|
Description: Rabbit Polyclonal NEMO/IKK-gamma Antibody. Validated in IP, WB and tested in Human. |
 Anti-PAK gamma Monoclonal Antibody |
|
A01419-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Mouse Monoclonal Antibody for PAK gamma Antibody (PAK2) detection. Tested with WB, IHC, IF in Human, Monkey. |
 Anti-CEBPG/Cebp Gamma Antibody |
|
A09205-1 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal CEBPG/Cebp Gamma Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
 Anti-HP1 gamma Monoclonal Antibody |
|
M01142-1 |
BosterBio |
100ug |
EUR 397 |
|
Description: Rabbit Monoclonal HP1 gamma Antibody. Validated in IP, WB and tested in Human, Mouse, Rat. |
 Anti-gamma Catenin/JUP Antibody |
|
PA1117-1 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-IKK gamma/IKBKG Antibody |
|
PA1551-1 |
BosterBio |
100ug/vial |
EUR 294 |
 MIP-3beta, murine recombinant protein |
|
P1049-.1 |
ApexBio |
100 µg |
EUR 877 |
|
Description: Macrophage inflammatory protein-3 ? (MIP-3?) is a CC chemokine produced in the thymus, lymph nodes and activated bone marrow stromal cells. MIP-3? signals through the chemokine receptor CCR-7/EBI-1/BLR-2. MIP-3? is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells. |
MIP-3beta, murine recombinant protein |
|
P1049-1 |
ApexBio |
1 mg |
EUR 5548 |
|
Description: Macrophage inflammatory protein-3 ? (MIP-3?) is a CC chemokine produced in the thymus, lymph nodes and activated bone marrow stromal cells. MIP-3? signals through the chemokine receptor CCR-7/EBI-1/BLR-2. MIP-3? is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells. |
 AssayMax ELISA Kit) Mouse Interferon-gamma (IFN-gamma) AssayMax ELISA Kit |
|
EMI1023-1 |
AssayPro |
96 Well Plate |
EUR 477 |
 Anti-Fibrinogen gamma chain/FGG Antibody |
|
A00790-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Anti-Interferon gamma Rabbit Monoclonal Antibody |
|
M00393-1 |
BosterBio |
100ug/vial |
EUR 397 |
|
Description: Rabbit Monoclonal Interferon gamma Antibody. Validated in Flow Cytometry, IF, ICC, WB and tested in Human, Mouse, Rat. |
 Anti-Catenin gamma Rabbit Monoclonal Antibody |
|
M01901-1 |
BosterBio |
100ug/vial |
EUR 397 |
|
Description: Rabbit Monoclonal Catenin gamma Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
 Anti-Interferon gamma Biotinylated Antibody |
|
A00393-1-Biotin |
BosterBio |
50ug/vial |
EUR 294 |
 Protein) Recombinant Human MIP-3 (CCL23) Protein |
|
PROTP55773-1 |
BosterBio |
20ug |
EUR 317 |
|
Description: MIP-3 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 is an 11.3 kDa protein containing 99 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
 Protein) Recombinant Murine MIP-2 (CXCL2) Protein |
|
PROTP10889-1 |
BosterBio |
20ug |
EUR 317 |
|
Description: All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant murine MIP-2 is a 7.8 kDa protein consisting of 73 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. |
 Anti-MIP-5 Antibody |
|
A05563 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for MIP-5 Antibody (CCL15) detection.tested for IHC in Human. |
 Anti-MIP-1b Antibody |
|
A16621 |
BosterBio |
100ul |
EUR 397 |
|
Description: Rabbit Polyclonal Antibody for MIP-1b Antibody (CCL4L1) detection. Tested with WB in Human. |
 Anti-MIP- beta antibody |
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STJ96572 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to MIP-1beta. |
 Anti-MIP-1b antibody |
|
STJ97347 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to MIP-1b. |
 Anti-MIP- alpha antibody |
|
STJ98775 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to MIP-3alpha. |
 Anti-MIP-5 antibody |
|
STJ98780 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to MIP-5. |
Anti-MIP- alpha antibody |
|
STJ94130 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to MIP-1alpha. |
Anti-MIP- beta antibody |
|
STJ94131 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to MIP-3beta. |
 Anti-MIP-T3 antibody |
|
STJ94133 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to MIP-T3. |
 anti- MIP-3α antibody |
|
FNab09791 |
FN Test |
100µg |
EUR 548.75 |
|
|
|
Description: Antibody raised against MIP-3α |
 Anti-strogen Related Receptor gamma/ESRRG Antibody |
|
A01470-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Polyclonal Goat anti-GST μ-form |
|
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 280 |
 Polyclonal Goat anti-GST p-form |
|
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 280 |
) MIP-1 beta, CCL4, murine (mouse) |
|
RC335-15 |
Bio Basic |
2ug |
EUR 104.38 |
|
|
 Protein) Recombinant Human MIP-3 Beta (CCL19) Protein |
|
PROTQ99731-1 |
BosterBio |
20ug |
EUR 317 |
|
Description: MIP-3β is a CC chemokine that is expressed in the thymus, lymph nodes and in activated bone marrow stromal cells and signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes and myeloid progenitor cells. Human MIP-3β is active on murine cells. Recombinant human MIP-3β is an 8.8 kDa protein containing 77 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
 Protein) Recombinant Murine MIP-3 Alpha (CCL20) Protein |
|
PROTO89093-1 |
BosterBio |
20ug |
EUR 317 |
|
Description: MIP-3α is a CC chemokine that is expressed in the liver, lymph nodes, appendix, PBL and lung and signals through the CCR6 receptor. MIP-3α is chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. Recombinant murine MIP-3α is a 7.9 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
 anti-AMPK gamma 1 |
|
YF-PA13985 |
Abfrontier |
100 ug |
EUR 403 |
|
Description: Rabbit polyclonal to AMPK gamma 1 |
 anti-Laminin gamma 1 |
|
YF-PA24068 |
Abfrontier |
50 ul |
EUR 334 |
|
Description: Mouse polyclonal to Laminin gamma 1 |
 MIP 1 alpha antibody |
|
20R-1788 |
Fitzgerald |
100 ug |
EUR 673 |
|
Description: Rabbit polyclonal MIP 1 alpha antibody |
 MIP 1 beta antibody |
|
20R-1789 |
Fitzgerald |
100 ug |
EUR 673 |
|
Description: Rabbit polyclonal MIP 1 beta antibody |
 MIP-1-alpha Antibody |
|
5246-100 |
Biovision |
|
EUR 338 |
 MIP-1-beta Antibody |
|
5249-100 |
Biovision |
|
EUR 338 |
 MIP-1 alpha Antibody |
|
DF8572 |
Affbiotech |
200ul |
EUR 304 |
|
Description: MIP-1 alpha Antibody detects endogenous levels of total MIP-1 alpha. |
MIP-1 alpha Antibody |
|
20-abx148975 |
Abbexa |
|
|
|
|
 MIP-1? Polyclonal Antibody |
|
ES7128-100ul |
ELK Biotech |
100ul |
EUR 279 |
|
|
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