VCF formatted files are the lingua franca of next-generation sequencing, whereas HL7 FHIR is rising as a commonplace language for digital well being document interoperability. A rising variety of FHIR-based scientific genomics functions are rising. Here, we describe an open supply utility for changing variants from VCF format into HL7 FHIR format. vcf2fhir converts VCF variants into a FHIR Genomics Diagnostic Report. Conversion interprets every VCF row into a corresponding FHIR-formatted variant within the generated report. In scope are easy variants (SNVs, MNVs, Indels), together with zygosity and section relationships, for autosomes, intercourse chromosomes, and mitochondrial DNA. Input parameters embody VCF file and genome construct (‘GRCh37’ or ‘GRCh38’); and optionally a conversion area that signifies the area(s) to convert, a studied area that lists genomic areas studied by the lab, and a non-callable area that lists studied areas deemed uncallable by the lab.
Conversion may be restricted to a subset of VCF by supplying genomic coordinates of the conversion area(s). If studied and non-callable areas are additionally provided, the output FHIR report will embody ‘region-studied’ observations that element which parts of the conversion area have been studied, and of these studied areas, which parts have been deemed uncallable. We illustrate the vcf2fhir utility by way of two case research. The first, ‘SMART Cancer Navigator’, is a net utility that provides scientific resolution assist by linking affected person EHR data to cancerous gene variants.
The second, ‘Precision Genomics Integration Platform’, intersects a affected person’s FHIR-formatted scientific and genomic information with information bases so as to present on-demand supply of contextually related genomic findings and proposals to the EHR. Experience to date exhibits that the vcf2fhir utility may be successfully woven into clinically helpful genomic-EHR integration pipelines. Additional testing can be a crucial step in the direction of the scientific validation of this utility, enabling it to be built-in in a number of actual world information movement situations. For now, we suggest using this utility primarily to speed up FHIR Genomics understanding and to facilitate experimentation with additional integration of genomics information into the EHR.
Implementation of Bio-Risk Management System in a National Clinical and Medical Referral Centre Laboratories
The rising threats from organic brokers have turn into a concern in laboratories, and rising infectious ailments have demanded elevated consciousness and preparedness of laboratory amenities. Bio-risk evaluation is required to present a framework for organisations to set up a complete bio-risk administration system. The evaluation standards ought to embody each biosafety and biosecurity measures. Laboratories in Indonesia play a important function in public well being interventions in time period of illness screening, analysis and medical resolution making. The National Clinical and Medical Referral Centre Laboratories have the potential of day by day exposures to harmful organic supplies.
This research goals to establish the hole between bio-risk administration system implementation and International Standard Organisation (ISO) 35001:2019 necessities. The 202 gadgets in ISO 35001:2019 are categorized into seven predominant components. The findings present that greater than half of the weather on ISO 35001:2019 have been applied in these centres. Good efficiency was recognized at lab Four and 5 which obtained the very best scores, notably within the context of organisation, planning, operation and enchancment components. However, the widest hole was present in management, assist and efficiency analysis. One means to deal with this could be to create written guidelines and laws on the laboratory high administration degree to require all laboratory amenities to comply to the bio-risk insurance policies, guidelines, and laws.
Out of the Lab and Into the Field: Validation of Portable Cell Culture Protocols
Field-based analysis on irritation and well being is usually restricted to baseline measures of circulating cytokines or acute section proteins, whereas lab-based research can pursue a extra dynamic strategy with ex vivo cell tradition strategies. The laboratory infrastructure required for culturing leukocytes limits utility in community-based settings, which in flip limits scientific understandings of how psychosocial, behavioral, and contextual components affect the regulation of irritation. We evaluated the efficiency of each protocols towards a commonplace laboratory-based protocol, utilizing matched venous and capillary blood samples collected from younger adults (N=24). Samples have been incubated with lipopolysaccharide (LPS) and hydrocortisone, and the manufacturing of pro-inflammatory cytokines IL-1β, IL-6 and TNFα was measured in response.
 ELISA Kit) Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 489 |
Mouse Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 677 |
 anti-LIF |
|
YF-PA12952 |
Abfrontier |
50 ug |
EUR 363 |
|
Description: Mouse polyclonal to LIF |
 ELISA Kit) Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-b-48T |
DL Develop |
48T |
EUR 547 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-b-96T |
DL Develop |
96T |
EUR 715 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
 ELISA Kit) Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-c-48T |
DL Develop |
48T |
EUR 527 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
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DLR-LIF-c-96T |
DL Develop |
96T |
EUR 688 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Canine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
 ELISA Kit) Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-Hu-48T |
DL Develop |
48T |
EUR 380 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-Hu-96T |
DL Develop |
96T |
EUR 485 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
 ELISA Kit) Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-p-48T |
DL Develop |
48T |
EUR 547 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-p-96T |
DL Develop |
96T |
EUR 715 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
 ELISA Kit) Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-Ra-48T |
DL Develop |
48T |
EUR 508 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
DLR-LIF-Ra-96T |
DL Develop |
96T |
EUR 661 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Leukemia Inhibitory Factor (LIF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 580 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 807 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 557 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 774 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 383 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 525 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 580 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 807 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 534 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RDR-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 742 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 555 |
Bovine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 771 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 533 |
Canine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 740 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 367 |
Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 502 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 555 |
Porcine Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 771 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 511 |
Rat Leukemia Inhibitory Factor (LIF) ELISA Kit |
|
RD-LIF-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 709 |
 Anti-LIF Antibody |
|
PA1562 |
BosterBio |
100ug/vial |
EUR 294 |
Anti-LIF Antibody |
|
PB9036 |
BosterBio |
100ug/vial |
EUR 334 |
Anti-LIF Antibody |
|
A01400 |
BosterBio |
100ug/vial |
EUR 294 |
 Anti-LIF antibody |
|
STJ96472 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Rabbit polyclonal to LIF. |
Anti-LIF antibody |
|
STJ29846 |
St John's Laboratory |
100 µl |
EUR 277 |
|
Description: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
 anti- LIF antibody |
|
FNab09804 |
FN Test |
100µg |
EUR 548.75 |
|
|
|
Description: Antibody raised against LIF |
 Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN |
ImmunoStep |
50 µg |
EUR 349 |
 Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 |
Detroit R&D |
50 uL |
EUR 280 |
 Polyclonal Goat anti-GST μ-form |
|
GST-ANTI-2 |
Detroit R&D |
50 uL |
EUR 280 |
 Polyclonal Goat anti-GST p-form |
|
GST-ANTI-3 |
Detroit R&D |
50 uL |
EUR 280 |
 Mouse LIF ELISA Kit |
|
EML0014 |
Abclonal |
96Tests |
EUR 521 |
 Mouse LIF shRNA Plasmid |
|
20-abx971321 |
Abbexa |
|
|
|
|
 Recombinant Mouse LIF Protein |
|
R01400-1 |
BosterBio |
100ug/vial |
EUR 294 |
 LIF Antibody |
|
25283-100ul |
SAB |
100ul |
EUR 390 |
LIF Antibody |
|
36950-100ul |
SAB |
100ul |
EUR 252 |
LIF Antibody |
|
1-CSB-PA198481 |
Cusabio |
|
|
|
|
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Description: A polyclonal antibody against LIF. Recognizes LIF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:10000, IHC:1:25-1:100 |
LIF Antibody |
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1-CSB-PA012928ESR2HU |
Cusabio |
|
|
|
|
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Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
LIF Antibody |
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1-CSB-PA004761 |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against LIF. Recognizes LIF from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
 LIF antibody |
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70R-6230 |
Fitzgerald |
50 ug |
EUR 467 |
|
Description: Rabbit polyclonal LIF antibody |
 LIF protein |
|
30R-2771 |
Fitzgerald |
10 ug |
EUR 369 |
|
Description: Purified recombinant Human LIF protein |
LIF protein |
|
30R-2772 |
Fitzgerald |
10 ug |
EUR 489 |
|
Description: Purified recombinant Mouse LIF protein |
LIF antibody |
|
70R-12299 |
Fitzgerald |
100 ug |
EUR 403 |
|
Description: Rabbit polyclonal LIF antibody |
LIF antibody |
|
70R-14100 |
Fitzgerald |
100 ug |
EUR 322 |
|
Description: Affinity purified Rabbit polyclonal LIF antibody |
LIF antibody |
|
70R-51295 |
Fitzgerald |
100 ul |
EUR 244 |
|
Description: Purified Polyclonal LIF antibody |
 LIF Peptide |
|
6245P |
ProSci |
0.05 mg |
EUR 164.75 |
|
Description: (IN) LIF peptide |
LIF Peptide |
|
43-200P |
ProSci |
0.1 mg |
EUR 338 |
LIF Antibody |
|
6245-002mg |
ProSci |
0.02 mg |
EUR 171.82 |
|
|
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
LIF Antibody |
|
6245-01mg |
ProSci |
0.1 mg |
EUR 436.42 |
|
|
|
Description: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation. |
 Mouse LIF PicoKine ELISA Kit |
|
EK0580 |
BosterBio |
96 wells |
EUR 425 |
|
Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA). |
 (OKBB00760)) LIF ELISA Kit (Mouse) (OKBB00760) |
|
OKBB00760 |
Aviva Systems Biology |
96 Wells |
EUR 505 |
|
Description: Description of target: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml |
 (OKEH04045)) LIF ELISA Kit (Mouse) (OKEH04045) |
|
OKEH04045 |
Aviva Systems Biology |
96 Wells |
EUR 544 |
|
Description: Description of target: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.039 ng/mL |
 ELISA kit for Mouse LIF |
|
EK5250 |
SAB |
96 tests |
EUR 553 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse LIF in samples from serum, plasma, tissue homogenates and other biological fluids. |
 LIF, Mouse Recombinant , 1MIU/ml |
|
L4500-001 |
GenDepot |
1ml |
EUR 339 |
LIF, Mouse Recombinant , 1MIU/ml |
|
L4500-005 |
GenDepot |
5x1ml |
EUR 1193 |
 LIF, Mouse Recombinant , 10MIU/ml |
|
L4501-001 |
GenDepot |
1ml |
EUR 1454 |
, mouse) Leukemia Inhibitory Factor (LIF), mouse |
|
PR16102 |
Neuromics |
5 ug |
EUR 435 |
 Human CellExp? LIF, mouse recombinant |
|
7268-10 |
Biovision |
|
EUR 370 |
 (pORF)) Lif ORF Vector (Mouse) (pORF) |
|
ORF049145 |
ABM |
1.0 ug DNA |
EUR 506 |
Lif ORF Vector (Mouse) (pORF) |
|
ORF049146 |
ABM |
1.0 ug DNA |
EUR 506 |
 LIF Polyclonal Antibody |
|
ES3837-50ul |
ELK Biotech |
50ul |
EUR 207 |
|
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
LIF Polyclonal Antibody |
|
ES3837-100ul |
ELK Biotech |
100ul |
EUR 279 |
|
Description: A Rabbit Polyclonal antibody against LIF from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
LIF Polyclonal Antibody |
|
ABP52838-003ml |
Abbkine |
0.03ml |
EUR 158 |
|
|
|
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF |
LIF Polyclonal Antibody |
|
ABP52838-01ml |
Abbkine |
0.1ml |
EUR 289 |
|
|
|
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF |
LIF Polyclonal Antibody |
|
ABP52838-02ml |
Abbkine |
0.2ml |
EUR 414 |
|
|
|
Description: A polyclonal antibody for detection of LIF from Human. This LIF antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human LIF |
 LIF Blocking Peptide |
|
20-abx064170 |
Abbexa |
|
|
|
|
 LIF Rabbit pAb |
|
A1288-100ul |
Abclonal |
100 ul |
EUR 308 |
LIF Rabbit pAb |
|
A1288-200ul |
Abclonal |
200 ul |
EUR 459 |
Comparisons point out a excessive degree of settlement in responses throughout the protocols and tradition circumstances. The total correlation in outcomes was 0.88 between the usual and small quantity protocols, and 0.86 between the usual and capillary blood protocols. Repeatability for the small quantity and capillary blood protocols was excessive, with imply coefficient of variation throughout 5 replicates of 6.2 and 5.4%, respectively.ConclusionsThese outcomes reveal the feasibility of culturing cells and quantifying the inflammatory response to problem outdoors the lab, with a wide selection of potential functions in biobehavioral analysis in community-based and distant subject settings.
