Rational choice of a biomarker panel focusing on unmet scientific wants in viral kidney damage
The pipeline of biomarker translation from bench to bedside is difficult and restricted biomarkers have been adopted to routine scientific care. Ideally, biomarker analysis and improvement ought to be pushed by unmet scientific wants in well being care. To information researchers, scientific chemists and clinicians in their biomarker analysis, the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) has developed a structured questionnaire in which the scientific gaps in present scientific pathways are recognized and fascinating efficiency specs are predefined. In kidney damage, the excessive prevalence of the syndrome acute kidney damage (AKI) in the hospital setting has a important influence on morbidity, affected person survival and well being care prices, however the use of biomarkers indicating early kidney damage in day by day affected person care stays restricted.
Routinely, medical labs measure serum creatinine, which is a purposeful biomarker, insensitive for detecting early kidney harm and can not distinguish between renal and prerenal AKI. The perceived unmet scientific wants in kidney damage had been recognized via the EFLM questionnaire. Nephrologists inside our tertiary care hospital emphasised that biomarkers are wanted for (1) early analysis of in-hospital AKI after a medical insult and in critically in poor health sufferers, (2) danger stratification for kidney damage prior to a scheduled (elective) intervention, (3) kidney damage monitoring in sufferers scheduled to obtain nephrotoxic medicine and after kidney transplantation and (4) differentiation between prerenal AKI and structural kidney harm.
The biomarker search and choice technique resulted in a rational choice of an eleven-protein urinary panel for kidney damage that concentrate on these scientific wants. To assess the scientific utility of the proposed biomarker panel in kidney damage, a multiplexed LC-MS check is now in improvement for the meant translational analysis. The rugged method detection limits, calculated from measurements in greater than 60 runs, for As, Cr, and Ni are 0.23 μg L-1, 0.19 μg L-1, and 0.31 μg L-1, respectively.
Different approaches to enhance cohort identification utilizing digital well being data: X-linked hypophosphatemia for example
Electronic Health Records (EHRs) symbolize a supply of excessive worth knowledge which is commonly underutilized as a result of exploiting the knowledge contained therein requires specialised strategies unavailable to the tip person i.e. the doctor or the investigator. Here I describe 4 easy and sensible avenues that can permit the usual EHR finish person to establish affected person cohorts: the use of diagnostic codes from completely different worldwide catalogues; a search in studies from complementary assessments (e.g. radiographs or lab assessments) for any consequence of curiosity; a free textual content search; or a drug prescription search in the affected person’s digital prescription document.
This medical method is buying nice significance in the sector of uncommon ailments, and right here I display its utility with X-linked hypophosphatemia. The use of these 4 EHR questioning approaches makes discovering a cohort of sufferers of any situation or illness possible and manageable, and as soon as every case document is checked, a well-defined cohort could be assembled.
Goat IgG-PE conjugate (isotype control) |
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20011-PE | Alpha Diagnostics | 100 tests | EUR 225 |
Rat IgG-Biotin conjugate (isotype control) (Isotype control) |
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20005-B | Alpha Diagnostics | 100 ug | EUR 164 |
Rat IgG-FITC conjugate (isotype control) (Isotype control) |
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20005-F | Alpha Diagnostics | 100 ug | EUR 164 |
Rat IgG-HRP conjugate (isotype control) (Isotype control) |
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20005-HP | Alpha Diagnostics | 100 ug | EUR 164 |
Rat IgG-PE conjugate (isotype control) (Isotype control) |
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20005-PE | Alpha Diagnostics | 25 tests | EUR 202 |
Goat IgG (fc)-Biotin conjugate (isotype control) |
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20011-Fc-B | Alpha Diagnostics | 100 ug | EUR 164 |
Goat IgG (fc)-FITC conjugate (isotype control) |
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20011-Fc-F | Alpha Diagnostics | 100 ug | EUR 164 |
Goat IgG (fc)-HRP conjugate (isotype control) |
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20011-Fc-HP | Alpha Diagnostics | 100 ug | EUR 164 |
Goat F(ab')2 IgG Isotype Control |
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abx405076-05mg | Abbexa | 0.5 mg | EUR 411 |
Rabbit IgG Isotype Control |
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abx125003-100ul | Abbexa | 100 ul | EUR 203 |
Rabbit IgG Isotype Control |
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IC001-100ul | SAB | 100ul | EUR 122 |
Mouse IgG Isotype Control |
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IC002-100ul | SAB | 100ul | EUR 122 |
IgG Isotype Control antibody |
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10R-6524 | Fitzgerald | 50 ug | EUR 133 |
Description: Armenian Hamster monoclonal IgG Isotype Control antibody |
IgG Isotype Control antibody |
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10R-6525 | Fitzgerald | 50 ug | EUR 133 |
Description: Syrian Hamster monoclonal IgG Isotype Control antibody |
Rat IgG Isotype Control |
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31-AR15 | Fitzgerald | 10 mg | EUR 220 |
Description: Purified Rat IgG Isotype Control |
Goat IgG (-ve control for flow cytometry) (isotype control) |
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20011-100 | Alpha Diagnostics | 100 test | EUR 103 |
Goat IgG, purified (serum non-immune, isotype control) |
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20011-1 | Alpha Diagnostics | 1 mg | EUR 141 |
Goat IgG, purified (serum non-immune, isotype control) |
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20011-25 | Alpha Diagnostics | 25 mg | EUR 469 |
Goat IgG, purified (serum non-immune, isotype control) |
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20011-5 | Alpha Diagnostics | 5 mg | EUR 286 |
Goat F(ab')2 IgG Isotype Control (FITC) |
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abx405077-100tests | Abbexa | 100 tests | EUR 495 |
Goat F(ab')2 IgG Isotype Control (RPE) |
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abx405078-05ml | Abbexa | 0.5 ml | EUR 537 |
Rat IgG purified (isotype control) |
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20005-1-200 | Alpha Diagnostics | 0.5 ml | EUR 103 |
Rat IgG, purified (Isotype control) |
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20005-5 | Alpha Diagnostics | 5 mg | EUR 286 |
Sheep IgG, purified (isotype control) |
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20006-1 | Alpha Diagnostics | 1 mg | EUR 141 |
Rabbit IgG-biotinylated (isotype control) |
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20009-BT-1 | Alpha Diagnostics | 1 mg | EUR 286 |
Hamster (Armenian) IgG Isotype Control |
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abx405028-025mg | Abbexa | 0.25 mg | EUR 384 |
Hamster (Arnenian) IgG Isotype Control |
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abx405029-05mg | Abbexa | 0.5 mg | EUR 592 |
Hamster (Syrian) IgG Isotype Control |
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abx405032-025mg | Abbexa | 0.25 mg | EUR 384 |
Syrian Hamster IgG Isotype Control |
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SIGGA-100 | ImmunoStep | 100 µg | EUR 574.16 |
Syrian Hamster IgG Isotype Control |
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SIGGB-200 | ImmunoStep | 200 µg | EUR 472.24 |
Syrian Hamster IgG Isotype Control |
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SIGGF-200 | ImmunoStep | 200 µg | EUR 545.04 |
Syrian Hamster IgG Isotype Control |
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SIGGPE-100 | ImmunoStep | 100 µg | EUR 515.92 |
Syrian Hamster IgG Isotype Control |
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SIGGPP5.5-100 | ImmunoStep | 100 µg | EUR 676.08 |
Syrian Hamster IgG Isotype Control |
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SIGGPP5.5-25 | ImmunoStep | 25 µg | EUR 355.76 |
Syrian Hamster IgG Isotype Control |
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SIGGPU-500 | ImmunoStep | 500 µg | EUR 428.56 |
Rabbit IgG Isotype control, Concentrate |
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NG910C | Innovex | 0.5 ml | EUR 392 |
Armenian Hamster IgG Isotype control |
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AIGGA-100 | ImmunoStep | 100 µg | EUR 574.16 |
Armenian Hamster IgG Isotype control |
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AIGGA-25 | ImmunoStep | 25 µg | EUR 282.96 |
Armenian Hamster IgG Isotype control |
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AIGGB-200 | ImmunoStep | 200 µg | EUR 472.24 |
Armenian Hamster IgG Isotype control |
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AIGGB-50 | ImmunoStep | 50 µg | EUR 239.28 |
Armenian Hamster IgG Isotype control |
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AIGGF-200 | ImmunoStep | 200 µg | EUR 545.04 |
Armenian Hamster IgG Isotype control |
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AIGGF-50 | ImmunoStep | 50 µg | EUR 268.4 |
Armenian Hamster IgG Isotype control |
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AIGGPE-100 | ImmunoStep | 100 µg | EUR 515.92 |
Armenian Hamster IgG Isotype control |
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AIGGPE-25 | ImmunoStep | 25 µg | EUR 253.84 |
Armenian Hamster IgG Isotype control |
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AIGGPP-100 | ImmunoStep | 100 µg | EUR 574.16 |
Armenian Hamster IgG Isotype control |
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AIGGPP-25 | ImmunoStep | 25 µg | EUR 268.4 |
Armenian Hamster IgG Isotype control |
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AIGGPP5.5-100 | ImmunoStep | 100 µg | EUR 646.96 |
Armenian Hamster IgG Isotype control |
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AIGGPP5.5-25 | ImmunoStep | 25 µg | EUR 282.96 |
Armenian Hamster IgG Isotype control |
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AIGGPU-50 | ImmunoStep | 50 µg | EUR 195.6 |
Armenian Hamster IgG Isotype control |
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AIGGPU-500 | ImmunoStep | 500 µg | EUR 428.56 |
FITC Rabbit IgG - Isotype control |
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DB1000 | DB Biotech | 100 tests | EUR 422 |
FITC Rabbit IgG - Isotype control |
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DB-1000 | DB Biotech | 100 tests | EUR 422 |
IgG Isotype Control antibody (FITC) |
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61R-1136 | Fitzgerald | 50 ug | EUR 165 |
Description: Armenian Hamster monoclonal IgG Isotype Control antibody (FITC) |
IgG Isotype Control antibody (PE) |
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61R-1337 | Fitzgerald | 200 ug | EUR 440 |
Description: Armenian Hamster monoclonal IgG Isotype Control antibody (PE) |
IgG Isotype Control antibody (PE) |
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61R-1338 | Fitzgerald | 50 ug | EUR 181 |
Description: Syrian Hamster monoclonal IgG Isotype Control antibody (PE) |
IgG Isotype Control antibody (biotin) |
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61R-1586 | Fitzgerald | 50 ug | EUR 165 |
Description: Armenian Hamster monoclonal IgG Isotype Control antibody (biotin) |
IgG Isotype Control antibody (biotin) |
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61R-1587 | Fitzgerald | 50 ug | EUR 165 |
Description: Syrian Hamster monoclonal IgG Isotype Control antibody (biotin) |
IgG Isotype Control antibody (allophycocyanin) |
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61R-1768 | Fitzgerald | 100 ug | EUR 349 |
Description: Armenian Hamster monoclonal IgG Isotype Control antibody (allophycocyanin) |
IgG Isotype Control antibody (allophycocyanin) |
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61R-1769 | Fitzgerald | 50 ug | EUR 219 |
Description: Syrian Hamster monoclonal IgG Isotype Control antibody (allophycocyanin) |
IgG Isotype Control antibody (FITC) |
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61R-1800 | Fitzgerald | 25 ug | EUR 205 |
Description: Rabbit polyclonal IgG Isotype Control antibody (FITC) |
Donkey IgG (Control, non-immune, isotype control) |
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20028-1 | Alpha Diagnostics | 1 mg | EUR 164 |
Goat IgG Control |
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GT15900 | Neuromics | 1 mg | EUR 166 |
Goat IgG control |
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31R-AG001 | Fitzgerald | 1 mg | EUR 127 |
Description: Goat Immunoglobulins Goat IgG control |
Rabbit IgG Isotype Control APC Conjugate |
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APCCON | AssayPro | 0.15 mg | EUR 261 |
Rabbit IgG Isotype Control FITC Conjugate |
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FITCCON | AssayPro | 0.15 mg | EUR 261 |
Mouse IgG Isotype Control PerCP Conjugate |
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MPERCPCON | AssayPro | 0.15 mg | EUR 261 |
Mouse IgG Isotype Control RPE Conjugate |
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MRPECON | AssayPro | 0.15 mg | EUR 261 |
Rabbit IgG Isotype Control PerCP Conjugate |
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PERCPCON | AssayPro | 0.15 mg | EUR 261 |
Rabbit IgG Isotype Control RPE Conjugate |
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RPECON | AssayPro | 0.15 mg | EUR 261 |
Mouse IgG Isotype Control APC Conjugate |
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MAPCCON | AssayPro | 0.15 mg | EUR 261 |
Mouse IgG Isotype Control FITC Conjugate |
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MFITCCON | AssayPro | 0.15 mg | EUR 261 |
Hamster IgG, purified (Syrian, isotype control) |
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20003-1 | Alpha Diagnostics | 1 mg | EUR 141 |
Hamster IgG, purified (Armenian, Isotype control) |
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20003-1AH | Alpha Diagnostics | 1 mg | EUR 263 |
Human IgG-HRP Conjugate (isotype control) |
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20007-1-HP | Alpha Diagnostics | 500 ug | EUR 202 |
Mouse IgG-Biotin conjugate (isotype control) |
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20008-B | Alpha Diagnostics | 50 Tests | EUR 164 |
Mouse IgG-Biotin conjugate (isotype control) |
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20008-BT-1 | Alpha Diagnostics | 1 mg | EUR 164 |
Mouse IgG-FITC conjugate (isotype control) |
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20008-F | Alpha Diagnostics | 100 tests | EUR 164 |
Mouse IgG-HRP conjugate (isotype control) |
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20008-HP | Alpha Diagnostics | 50 Tests | EUR 164 |
Mouse IgG-PE conjugate (isotype control) |
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20008-PE | Alpha Diagnostics | 50 Tests | EUR 164 |
Rabbit IgG-FITC conjugate (isotype control) |
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20009-F | Alpha Diagnostics | 100 ug | EUR 164 |
Rabbit IgG-HRP conjugate (isotype control) |
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20009-HP | Alpha Diagnostics | 100 ug | EUR 164 |
Rabbit IgG-PE conjugate (isotype control) |
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20009-PE | Alpha Diagnostics | 100 tests | EUR 225 |
Hamster (Arnenian) IgG Isotype Control (FITC) |
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abx405030-01mg | Abbexa | 0.1 mg | EUR 384 |
Hamster (Arnenian) IgG Isotype Control (RPE) |
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abx405031-01mg | Abbexa | 0.1 mg | EUR 509 |
Hamster (Syrian) IgG Isotype Control (FITC) |
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abx405033-01mg | Abbexa | 0.1 mg | EUR 398 |
Donkey IgG (non-immune, isotype control) IgG, purified |
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20015-10 | Alpha Diagnostics | 10 mg | EUR 286 |
Llama IgG (Control, non-immune, isotype control, ELISA grade) |
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20030-1 | Alpha Diagnostics | 1 mg | EUR 164 |
Camel IgG (Control, non-immune, isotype control, ELISA grade) |
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20031-1 | Alpha Diagnostics | 1 mg | EUR 164 |
Alpaca IgG (Control, non-immune, isotype control, ELISA grade) |
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20032-1 | Alpha Diagnostics | 1 mg | EUR 164 |
Goat IgG Control Sera |
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20011-250 | Alpha Diagnostics | 0.5 ml | EUR 103 |
Hamster IgG-Biotin conjugate, isotype control (Syrian) |
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20003-1-B | Alpha Diagnostics | 100 test | EUR 164 |
Hamster IgG-Cy5 conjugate, isotype control (Syrian) |
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20003-1-Cy5 | Alpha Diagnostics | 50 tests | EUR 225 |
Hamster IgG-FITC conjugate, isotype control (Syrian) |
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20003-1-F | Alpha Diagnostics | 100 tests | EUR 164 |
Hamster IgG-HRP conjugate, isotype control (Syrian) |
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20003-1-HP | Alpha Diagnostics | 100 tests | EUR 164 |
G. Pig IgG-Biotin conjugate (isotype control) |
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20004-B | Alpha Diagnostics | 100 ug | EUR 202 |
G. Pig IgG-FITC conjugate (isotype control) |
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20004-F | Alpha Diagnostics | 100 ug | EUR 202 |
G. Pig IgG-HRP conjugate (isotype control) |
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20004-HP | Alpha Diagnostics | 100 ug | EUR 202 |
Rat IgG Fab fragment, purified (isotype control) |
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20005-1-FAB | Alpha Diagnostics | 1 mg | EUR 164 |